934 Comparative analysis of the skin microbiota of rosacea and rosacea-like diseases

Rosacea (RO), rosacea-like dermatitis (RL： also known as corticosteroid-induced rosacea), and perioral (PE) cause erythema, red papules, pustules on the face. Although causes of these diseases are unknown, tetracycline therapy is effective in some cases, suggesting involvement bacteria. To clarify etiologic significance skin microbiota diseases, we examined RO, RL, PE, well patients with acne vulgaris (AC), atopic (AD), seborrheic (SD) lesions, healthy volunteers (NL) (n=72), using swab specimens that were obtained by abrasion surface, soft hair collected from lesions. For analysis, DNA was extracted specimens, PCR products 16S rRNA gene (V1-V2 region) made into a library, amplicon analysis performed to obtain data. Comparative at initial diagnosis each disease revealed bacterial composition similar for RL but differed significantly other NL. In unclassified Proteobacteria predominant PE cases. The family Neisseriaceae abundant surface RO Furthermore, investigated change during course treatment PE. after antibiotic therapy, disappeared improvement rash. These results indicate has significant impact pathogenesis microbiota-targeted